Oloa
Hes; cay leai .:7365-45-9
Uiga tutusa: 1- [4- (2-Hydroxythyl) -1-paipa-plainl] 2-sulfonacic-pyhousthyfyfy acid); n- (2-hydroxythyl) piperazine-n'-2-ethanesfulfmicyf-\-hydroythyfy ACID); n'-2-hydroxyethylpizine-n-2-e-2-Ethonecmicce-pyoraythyfyf;
Vailaʻau vailaʻau o hepe
● foliga / lanu: paʻepaʻe paʻepaʻe paʻepaʻe efuefu
● Vapar faʻamalosi: 0pa i le 25 ℃
● liusuavai tulaga: 234-238 ° C
● Faʻasaina le Faʻailoga: N20 / D 1.339
● Bloiling Point: 408 ℃ [i le 101 325 pa]
● pka: 7.5 (i le 25 ℃)
● asa:89.46000
● O le DENESE: 1.325 G / CM3
● Logp: -0.559930
● Teu Temp.:27-8
● sonelity.:h2o: 1 m i le 20 ° C, manino, lanu, leai se lanu
● vai sonebersity.: -Sable
● XLLLPP3: -4.1
● Hydrogen Bond Dainon Count: 2
● Hydrogen Bond Reportor faitau: 5
● Fuga o le Bonduble Bond Rep: 4
● sooga tele: 238.09872823
● mamafa atom faitau: 15
● Faigofie: 254
Faamatalaga o le Safty
● Pikigram (s):
Xi
● lamatiaga numera: xi
● Faʻamatalaga: 36/37/38
● Faʻamatalaga saogalemu: 24 / 25-22-36-26-26
Aoga
Ataata ataata:C1cn (CC [NH +] 1ccs (= o) (= o) [O - o -]) CCO
Faamatalaga:O HEPs na faʻamatala o se tasi o sili sili ona lelei-mafuaʻaga buffits o loʻo avanoa mo suʻesuʻega a le biological. I le Biolog Ph, o le mole o le Zwitterricac, ma e aoga o se buffer i le ph 6.8 i le 8.2 (PK 7.55). E masani ona faʻaaogaina i le sela aganuʻu i le mafaufau loloto i le va o 5mm i le 30 mm. HEpes ua faʻaaogaina i se tele ituaiga o talosaga, e aofia ai ma le amio faʻafanua. E masani ona faʻaogaina i totonu o le Burffer Cell Coupinity Faʻasalalauga i le ea. E maua ai e HEpe lona faʻaaogaina i totonu o VITO faʻataʻitaʻiga i le mg.
Faaaogaina:Leai se mea oona i le sela. E faʻaaogaina o se hydrogen ion buffer, e mafai ona pulea le tumau o le pH i luga o le taimi umi mo se taimi umi. O le mafaufau o le 10-50mmol / l. E masani lava, 20mmol / lhepes i Forthent Fofo e mafai ona maua ai le palu. Boity buffer. HEPs e masani ai buffer mo siama oona, aemaise faʻaaogaina i le sela aganuu e faʻatumauina le physiological ph. O loo galue ai se vaega o le vaʻalele, o loʻo faʻaaogaina i le tapenaina o paffer. O loʻo faʻamatala o se tasi o sili sili ona lelei-faʻamoemoe e avanoa mo le faʻaaogaina i totonu o le bioliman stories, o loʻo faʻaaoga i totonu o le tino o le hasidrat.
Faʻamatalaga auiliili
Hepetu mo le 2- (4- (2-hydroxyedyl) piperazin-1-yl) Ethoilingfoonic acid. O se vaʻalele e masani ona faʻaaogaina e masani ai i totonu o le tino ma le biochemical suʻesuʻega. HEPS SUʻESUʻI MAʻOAʻIGA O LE POTO I LE CHEC CHOCTERS CARD CARDS, AE mafai ona talia uma ma foaʻi protons, o loʻo faʻamaonia ai ni suiga o le PL. O lona vailaʻau fausaga ma meatotino e sili ona aoga i le faʻatumauina o le tumau pea o le tumau ma sili ona lelei ph mo le tele o le tino ma enzymatic talitane. HEBs e masani ona faʻaogaina i totonu o le aganuu aganuu, faʻamaliega o vailaʻau, ma le Molecular Biology System.
Talosaga
E i ai le pule o Hispes i nofoaga eseese. O nisi o le masani talosaga o hepes o:
Cel City: E masani ona faʻaaogaina le HEPs o se vaega i le sela aganuu faʻasalalau e tausia ai le PHED PHORTER. E fesoasoani e faʻatonutonuina le Ph Sui na mafua mai i gaioiga a le Gadabolic ma le faʻaputuputu o otaota oloa i totonu o le sela aganuu.
Enzyme e faamaoni:E masani ona faʻaaoga le HEPs o se faʻatauga a le sooupu i le enzyme e faʻatumauina le tumau o le ph, lea e taua tele mo le faʻaaogaina tatau o le enzymes. E fesoasoani ia mautinoa le saʻo ma le toe gaosia faʻaiuga.
Electrophoris:O le HEPs o loʻo faʻaaogaina o se vaʻalele o le sooupu i le eletise eletise e pei o Polyacrylade Gel Collectophoressis (itulau) E fesoasoani i le tausisia o le PH PHEVER mo le sili ona lelei o le vavaeeseina ma le auiliiliga o macromoleculas pei o porotini ma anickic amo.
Faamautu le Poronan:O le HEPs o nisi taimi e aofia ai i le porotini le faʻamamaina e tausia ai le manaʻomia o le i taimi o le sili atu laasaga o le faʻamatuʻuina. E fesoasoani i le mautu ma le gaioiga o porotini i le taimi o le faamamaina.
Molecular biogy metotia:HEPS e mafai ona faʻaaogaina i totonu o le tele o le tele o le tele o aʻoga biogy biology, pei o le PCR (Polymerase filifili filifili) ma le DNA o le PH. E fesoasoani e faʻalelei le faʻatinoga o lenzymes ma faʻamautinoaina mea saʻo ma faʻatuatuaina.
E taua ona iloa o le mafaufau faʻapitoa o le teteʻe ma lana talosaga e ono se ese faʻalagolago i tulaga faʻataʻitaʻi ma manaʻoga faʻapitoa.
