Oloa
HEPES sodium masima
Synonyms: HEPES sodium masima;75277-39-3;HEPES hemisodium salt;103404-87-1;Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;sodium 2-[4-(2-hydroxyethyl) )piperazin-1-yl]ethanesulfonate;HEPES (sodium);C8H17N2NaO4S;4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid hemisodium masima;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, monosodium salt;4-(2-Hydroxyethyl)piperazine-1 ethanesulfonic acid sodium masima;Sodium 4-(2-hydroxyethyl)piperazin-1-ylethanesulphonate;UNII-Z9FTO91O8A;Z9FTO91O8A;sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate;DTXSID4044458;CHEBI:46758;CHEBI:46758 278-169-7;1-Piperazineethanesulfonic acid, 4-(2-hydroxyethyl)-, sodium masima (1:1);N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid,sodium masima;HEPES sodium masima, >=99.5% (titration);sodium hepes;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid) sodium masima;MFCD00036463;HEPES, sodium masima;HEPES sodium masima, 98%;C8H18N2O4S.Na;SCHEMBL229142;WAS-14;CHEMBL3187284;DTXCID2024458;RDZTWEVXRGYCFV-UHFFFAOYSA-M;C8-H18-N2-O4-S.Nalt solution sodium;HEPES; (1M);Tox21_302155;HB5187;N-(2-Hydroxyethyl)piperazine-N-(2-ethanesulfonicacid)hemisodiumsalt;HEPES sodium masima, biochemical vasega;AKOS015897769;AKOS015912229;AKOS015964204;NCGC00255791-01;CAS-75277-39-3;HY-108535;CS-0029103;FT-06108368;F2A8319; N'-hydroxyethyl-N-piperazineethanesulfonate;HEPES sodium masima, BioXtra, >=99.0% (titration);HEPES sodium masima, Vetec(TM) reagent grade, 96%;W-104397;Q27120698;Sodium2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate;4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid sodium masima;4-(2-hydroxyethyl)- 1-piperazineethanesulfonic acid, monosodium masima;N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)sodiumsalt;N-2-Hydroxyethylpiperazine-N'-2-ethanesulphonic acid hemisodium salt;HEPES sodium salt, BioPerformance Certified, talafeagai mo aganuu cell, >=99.5%
Meatotino Vailaau o le HEPES Sodium Salt
● Faʻaaliga / Lanu: paʻepaʻe paʻepaʻe
● Ausa oona: 0Pa i le 25 ℃
● Lisi Liu:234 °C
● PKA:7.5(i le 25℃)
● PSA：92.29000
● Malosi:1.504[i le 20℃]
● LogP:-0.90190
● Temp. Teuga: Teu i RT.
● Ma'ale'ale.:Hygroscopic
● Solubility.:H2O: 1 M i le 20 °C, manino, leai se lanu
● Suavai Solubility.:E solu i le vai.
● Faitauga o Fomai Fomai Hydrogen:1
● Faitauga o le Talia o Fa'amau:6
● Faitauga o Nonoa Liliu:5
● Misasa'o:260.08067248
● Faitauga Atomo mamafa:16
● Lavelave:272
Fa'amatalaga Saogalemu
● Pictogram(s):
● Laiti o lamatiaga:
● Faamatalaga Saogalemu:22-24/25
E aoga
Vasega vaila'au:Isi Fa'aoga -> Biological Buffers
ATAITAU fa'aanonikala:C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]
Fa'aoga:Ole vaifofo masima ole HEPES e talafeagai mo su'esu'ega tu ma aga. E mafai ona fa'aoga ile tapenaga ole vaifofo pa'u ole HEPES. E mafai ona faʻaaogaina e fai ma paʻu i le taimi o suʻesuʻega o le phosphorylation i fibroblast faʻafefe. E mafai ona fa'aaoga e fai ai se fa'amama fa'amama toto lavelave, e fa'aaoga i le su'esu'eina ole thyroxin maua fua ile sua e ala ile radioimmunoassay. Ole vaifofo masima ole HEPES e talafeagai mo le fa'aoga ile su'esu'ega tu ma aga. E mafai ona fa'aoga ile tapenaga ole vaifofo pa'u ole HEPES. E mafai ona faʻaaogaina e fai ma paʻu i le taimi o suʻesuʻega o le phosphorylation i fibroblasts faʻafefe. E mafai ona fa'aaoga e fai ai se fa'amama fa'amama toto lavelave, e fa'aaoga i le su'esu'eina ole thyroxin maua fua ile sua e ala ile radioimmunoassay.
Fa'amatalaga Fa'atomuaga
HEPES sodium masima, e lauiloa foi o le 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt, o se faʻaoga masani ole HEPES. O le zwitterionic organic compound lea e galue e fai ma sui fa'apolopolo i su'esu'ega fa'aola ma biochemical.
Ole masima ole HEPES e masani ona fa'aogaina ile fa'asalalauga fa'aleaganu'u fa'aola ma fa'apolopolo ola olaola ona o lona gafatia e fa'amautu le tulaga o le pH fa'atatau ile tulaga ole tino (pH 7.2 - 7.6). E sili ona solu i le vai ma e maualalo le oona, e talafeagai ai mo fa'aoga eseese.
Ole masima masima ole HEPES e sili ona lelei ile tele o talosaga aua e faʻaleleia ai le solubility ma le mautu, faʻatusatusa i le fua ole acid form. O lo'o maua fa'apisinisi e pei o se pa'epa'e tioata pa'epa'e lea e faigofie ona fa'amuta i le vai e saunia ai fofo galue.
E masani ona fa'aogaina e le au su'esu'e le masima ole HEPES i le molecular biology techniques, cell culture, enzyme assays, protein purification, ma isi su'esu'ega biochemical lea e taua tele ai le pulea o le pH. O lona malosi fa'apalepale ma le fetaui lelei ma faiga fa'aola ua avea ai ma filifiliga ta'uta'ua mo le fa'atumauina o tulaga sili ona lelei mo le gaioiga ma le mautu o moleola ola eseese.
A'o le'i fa'aogaina le HEPES sodium salt i so'o se fa'aoga fa'apitoa, e mana'omia le toe iloilo o tusitusiga, fa'atalanoa fautuaga a le au fa'atau oloa, ma fa'amalieina le fa'atonuga ma le pH fa'atatau i mana'oga fa'ata'ita'i.
Fa'atatauga
Ole masima ole HEPES ole su'esu'e ua maua le fa'aogaina lautele i vaega eseese o meaola ma su'esu'ega fa'aola. O nisi o ana faʻaoga autu e aofia ai:
Aganu'u Cell:HEPES sodium masima e masani ona fa'aopoopo i fa'asalalauga fa'aleaganu'u sela e fa'amautu ai le pH mautu i totonu ole vaega ole physiological ma maua ai se si'osi'omaga talafeagai mo sela e ola ma fa'ateleina.
Sui Fa'amau:HEPES sodium masima e masani ona fa'aaogaina e fai ma sui fa'apolopolo i fa'aolaola ma vaifofo, e aofia ai su'esu'ega o le enzyme, fa'amamaina o porotini, ma metotia mole mole. E fesoasoani i le faatumauina o se pH tumau e ala i le tetee atu i suiga i le acidity poʻo le alkalinity.
Electrophoresis: HEPES sodium masima o loʻo faʻaaogaina e fai ma paʻu i gel electrophoresis e faʻamautu ai se pH mautu i le taimi o le vavaeeseina o nucleic acids poʻo polotini. E puipuia ai suiga i le pH e mafai ona aʻafia ai le femalagaiga ma le vavae ese o molelaʻau i le gel.
Polotini mautu: HEPES sodium masima ua fa'aopoopo i vaifofo polotini e fa'amautu ai lo latou fausaga ma fa'atumauina a latou gaioiga. E fesoasoani i le faatumauina o le siosiomaga sili ona lelei o le pH e manaʻomia mo le faʻamautuina o le porotini ma le faʻatinoga.
Gaoioiga Enzyme: HEPES sodium masima o lo'o fa'aaogaina e fai ma pa'u i gaioiga fa'a-enzymatic e fa'amautu ai le pH mana'omia mo le gaioiga sili ona lelei ole enzyme. E fesoasoani e faʻamautinoa o loʻo galue enzymes i lo latou maualuga maualuga ma le saʻo.
Fa'ata'ita'i Va'aiga Ola:HEPES sodium masima e masani ona aofia i totonu o faʻasalalauga ata mo suʻega faʻataʻitaʻiga ola. O lona malosi fa'apolopolo e fesoasoani e fa'atumauina le pH mana'omia ma puipuia ai suiga e ono a'afia ai le ola ma le fa'alelei o sela.
Molecular Biology Techniques: HEPES sodium masima o lo'o fa'aaogaina lautele i metotia mole mole biology, e aofia ai DNA po'o RNA tu'ufua, PCR, fa'asologa o DNA, ma su'esu'ega polotini. O lona gafatia fa'apolopolo e mafai ai ona maua fa'ai'uga sa'o ma fa'atuatuaina a'o fa'agasolo nei faiga.
E taua le maitauina o le faʻaogaina ma le faʻaogaina o le masima sodium HEPES e mafai ona fesuisuiaʻi e faʻatatau i manaʻoga faʻataʻitaʻi ma le faiga o meaola o loʻo suʻesuʻeina. O le mea lea, e fautuaina e fa'afeso'ota'i tusi ma fautuaga a le au fa'atau oloa mo le fa'aoga lelei.
